mouse testicular tm3 cell line  (Korean Cell Line Bank)

Journal: Molecular Metabolism

Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet

doi: 10.1016/j.molmet.2026.102350

Figure Lengend Snippet: AMPK inhibition-induced Angiogenin is accompanied by alteration of tRFs profile in vitro. A Western blot results of proteins related with AMPK-mTOR pathways in TM3, TM4, and NIH/3T3 cell lines using compound C (CC), rapamycin (Rapa), and MHY1485 (MHY) in TM3, TM4, and NIH/3T3 cell lines (left to right). The number represents the densiometric comparison of the expression of pAMPK and pmTOR proteins against AMPK and mTOR, respectively. B Angiogenin gene expression. Gene expression was quantified after 12 h of treatment. mRNA expression level was normalized to Actin . Data are represented as mean ± standard deviation. Statistical analysis was performed using One-way ANOVA with Tukey post-hoc test. Different letters are significantly different. C Northern blot results of tRF3-Pro and Val in mouse testis cell lines. Each sample RNAs were extracted from both TM3 and TM4 cells with CC treatment and overexpression of Angiogenin (Ang OE). Each intact tRNA was shown as a control. Band intensities of Northern blot are represented as bar graph after quantification using Image J software. Data are presented as mean ± standard deviation. One-way ANOVA was used for statistical analysis, and the different alphabets with Tukey post-hoc test. D Distribution of read counts based on small RNA type. E Distribution of reads assigned to GtRNAdb in tRF subtypes. The total bar height is proportional to the total read count for each group. Percentages indicate the relative proportion of each tRF subtype within the corresponding group, and n represents the actual read count number for each subtype. tRF5, reads derived from tRNA 5′ end among reads aligned to GtRNAdb; tRF3, reads derived from tRNA 3′ end among reads aligned to GtRNAdb; other, not included in the previous two groups. F Histogram showing the distribution of log 2 (Fold change) using reads aligned to GtRNAdb. Green, CC-treated versus non-treated; Yellow, ANG overexpression versus control. G Volcano plot depicting significantly changed tRFs in TM4 cell lines. Results from CC-treated versus non-treated are represented as circles, and results from ANG overexpression versus control are represented as triangles. n = 2 for each group.

Article Snippet: The mouse testicular TM3 cell line and TM4 Sertoli cell line were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Inhibition, In Vitro, Western Blot, Comparison, Expressing, Gene Expression, Standard Deviation, Northern Blot, Over Expression, Control, Software, Derivative Assay

Journal: Endocrinology

Article Title: Mouse testicular Leydig cells express Klk21, a tissue kallikrein that cleaves fibronectin and IGF-binding protein-3.

doi: 10.1210/endo.142.11.8505

Figure Lengend Snippet: FIG. 9. Effects of hormones on the expression of the mKlk21 gene in Leydig cells. Testicular Leydig cells isolated from 2-wk-old mice and TM3 Leydig cells were treated with various hormones at 107 M for 48 h. Total RNA from the treated cells was subjected to RT-PCR, followed by Southern blot analysis. The lower panel depicts the ex- pression of -actin as a control. Five animals were used for one ex- periment. Experiments were conducted twice to confirm the repro- ducibility of the results.

Article Snippet: The mouse testicular cell line TM3 (Leydig cells) was obtained from American Type Culture Collection (Manassas, VA).

Techniques: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction, Southern Blot, Control

Journal: The Journal of biological chemistry

Article Title: G protein-coupled receptor kinase GRK4. Molecular analysis of the four isoforms and ultrastructural localization in spermatozoa and germinal cells.

doi: 10.1074/jbc.272.15.10188

Figure Lengend Snippet: FIG. 5. Northern blot analysis of GRK4 mRNA. Total RNA (20 mg) from mouse Leydig cells TM3 (L), Sertoli cells TM4 (Se), spermatogonia GC-1 spg (Sp), and testis (Te) (left) and from 20- or 60-day-old rat testis (right) was hybridized with a GRK4 human cDNA probe common to the four isoforms. Washed filters were exposed at 280 °C for 24–48 h. Data represent two separate experiments.

Article Snippet: The mouse testicular cell lines TM3 (Leydig cells), TM4 (Sertoli cells), and GC-1 spg (spermatogonia) were from ATCC, and they were grown according to the manufacturer instructions.

Techniques: Northern Blot